 Wuhan EIAab Science Co., Ltd.
Contact info
name: phone: cell phone:
Email address:
Institute:
address:
Product Information
1. Purchase Information:
product name
Catalog# Lot#
distributor name
Order date:
Received date:
2. storage upon receiving the product:
ambient ice pack dry ice
REAGENT INFORMATION
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Item |
Lot number |
Reconstitution Buffer |
Reconstitution volume |
Reconstitution date |
Storage temperature |
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Capture Antibody |
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Detection Antibody |
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Standard |
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Plates
Plate type: _________
Manufacturer: _______
Catalog# _________
Blocking Buffer
Buffer components:
BSA:
Vendor name: _________
Catalog# ___________
Description:
Fraction V: Yes or No
Protease free: Yes or No
Date Prepared:
Reagent Diluent
Buffer components:
Date made:
Wash Buffer:
Components:
Method used to wash plate:
Number of washes:
Date wash prepared:
Streptavidin-HRP (if applicable)
Manufacturer:
Description:
Expiration Date:
Substrate
Manufacturer
Description:
Expiration date:
Wavelength Used:
Plate reader
Vendor:
Model#
ELISA Information:
- Problem observed? ____________________________________________________
- Sample type
Cell Culture: If Yes what type of cells and condition media used?
____________________________________________________
- Any positive control or negative control used? ________
- Are other assays using same secondary/chromogen system, performed on the same day working?
5. Working Concentrations
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Item |
Diluting Buffer |
Working Concentration |
Incubation Time |
Incubation Temperature |
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Capture antibody |
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Detection Antibody |
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Standard |
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Wash:
Describe method of wash:
Technical Notes
- Always follow the protocol given. Please note that not all the reagents diluents for all the matched antibody pairs and Duosets are the same.
- Thorough and consistent washing is required for proper assay performance. Wash solution should be dispensed forcefully and completely removed. Use of a multichannel pipettor for washing is not recommended. If a well maintained plate washer or multichannel manifold is not available, use a wash bottle. Insufficient washing can be a source of reproducibility problems.
- Store the components as directed.
- Use of fresh buffers and diluents is recommended. Please be aware that DuoSeTs and MAPs have been tested using substrates and stop solutions specified in the General ELISA Protocol and specific product inserts. Substitution of these components requires further optimization including kinetic testing.
- Use fresh plate sealers, pipette tips, and reagent troughs for each step.
- Please note that the recommended plate and BSA is indicated on the back of each Duoset insert.
- Data reduction may alter results. First, use the method recommended in the General ELISA protocol.
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Wuhan EIAab Science Co.,Ltd.