 Wuhan EIAab Science Co., Ltd.
Contact info name: phone: cell phone:
Email address:
Institution:
address:
Product Information
1. Purchase Information:
product name Catalog# Lot#
distributor name
Order date:
Received date:
2. Condition upon receiving the product):
ambient_____ ice pack_____ dry ice_____
package intact?: Inside___ Outside_____
bottles/vials leaking?: _____ any wetness/liquid______
kit components in appropriate condition?:
A. Reagents not unusually discolored or clouded? ______
B. Plate sealed completely? ______
Desiccant present and intact upon opening plate bag? ______
C. Lyophilized standard shows no sign of moisture? ______
D. All component part numbers match those listed in package insert? If not what are the part numbers? ___________
E. Any components expired?_______
3. storage after reconstitution:
ambient_____40C_____-200C_____ -700C_____
Observation
ELISA Assay Information
_____1. Read package insert accurately and performed all assay steps as outlined?
______ 2. Used proper matching kit components?
Did not use components from another kit?
Did not substitute with any non-R&D material?
______3. Components at room temperature before used?
______4. All components, solutions, samples, etc. prepared as recommended and mixed gently to homogeneity?
___________A. Proper diluents used?
(non-serum or serum calibrator diluent; assay diluent needed or not)?
___________B. Samples used: EDTA Plasma, Heparin Plasma, Urine, Cell Culture supernate, Serum, Tissue homogenate (indicate tissue type), Cell Lysate (indicate cell type and provide a recipe for the cell lysis buffer).
___________C. Performed proper reconstitutions of relevant components?
__________ D. Performed proper dilution schemes for standard, samples, etc.?
___________E. Used unique reservoirs for each reagent?
___________F. Unique pipettes and/or tips used per reagent or sample?
_______5. Plate tapped gently when necessary to ensure mixing of components after addition?
_______6. Plate sealers on tight?
_______7. Proper incubation time and temperature used?
_______ Plate not subjected to extreme or fluctuating temperature or light source?
_______8. Wash solution prepared properly with D.I. water?
______ Recommended wash technique used?
_______9. Color solution prepared as outlined
(1:1 mixture of A & B no more than 15 min. before use)?
______10. Stop solution added to wells in same order as color solution?
After addition of stop solution, were the wells green or yellow?
______11. Plate read within 30 min. after addition of stop?
________ Read at 450 nm?
__________Was correction wavelength used? If yes please indicate what was the correction wavelength used at?
______12. Data reduced using recommended curve fit (log-log or 4PL)?
Problems and Notes
Equipment Information
_____1. All relative measuring devices calibrated properly?
__________A. Pipettes?
__________B. Graduated cylinder?
__________C. Eight channel pipetter?
__________D. Plate shaker?
__________E. Timer?
__________F. Plate reader?
I. Vendor and model number ___________
II. Contains proper filters for required wavelengths?
III. Calibrate?
IV. Proper detectable optical density range to 3.0 OD units?
______2. Wash apparatus clean and functioning properly?
Type used: (squirt bottle)__, (manifold)__, (automated washer)___
If using automated washer, are you the sole user of it? If not which assay was run prior to yours?
______3. All glassware and/or plastic ware clean?
Observations
please supply all relative raw, non-zero-corrected OD data and assay templates.
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Wuhan EIAab Science Co.,Ltd.