 Wuhan EIAab Science Co., Ltd.
Contact info
name: phone: cell phone:
Email address:
Institute:
address:
Product Information
1. Purchase Information:
product name
Catalog# Lot#
BSA(Carrier-Free):
distributor name
Order date:
Received date:
2. storage upon receiving the product):
ambient ice pack dry ice
3. Reconstitution date reconstitution volume
Reconstitution buffer
4. storage after reconstitution:
00C ; 40C ; -200C ;-700C ;
Western blot Information
1. Type of gel: DTT/b-mercaptoethanol in loading buffer Yes___ No___
acrylamide（%）
2. Sample Type: sample amount per lane:
If samples are cell extract or Tissue extract, please provide a detail extraction protocol.
3. Positive control
4. Sample buffer(loading buffer) formulation:
5. Secondary antibody: label
vendor cat.#
secondary antibody used with other primary antibody:
name of other primary antibody
vendor, cat.#
6. Transfer apparatus:
Transfer buffer formulation:
Membrane Type
Transfer time Current (mA/cm2)
7. Method of protein determination
8. Staining protocol
blocking buffer: dry milk or BSA % ; [NaCl conc.]_____mM;
incubation (temp., time)
primary antibody conc.
Incubation (temp., time)
secondary antibody conc.
incubation (temp., time)
enzyme conc. Incubation (temp., time)
substrate conc. Incubation (temp. time)
vendor of substrate
9. Dot Blot performed?
10. Any other method used to confirm the presence or expression of the protein in the samples?
Please provide a copy of the gel with the molecular markers included, clearly label each lane.
Problems
3 | 
Wuhan EIAab Science Co.,Ltd.